Purification of three forms of lipocortin from bovine lung
NC Khanna, M Tokuda, DM Waisman - Cell Calcium, 1987 - Elsevier
Experimental conditions are described for simultaneous purification of three forms of
lipocortin (lipocortin I, lipocortin II and lipocortin-85) from bovine lung. The procedure yields
milligram quantities of all three lipocortins. Using antisera against lipocortin I and lipocortin
II, purified proteins show no cross contaminations. All forms of lipocortin exhibit equal
potency as in vitro bovine pancreatic phospholipase A 2 inhibitors. Protein kinase C
catalyzes the in, vivo incorporation of about 1.0, 0.7 and 0.4 mole of phosphate per mole of …
lipocortin (lipocortin I, lipocortin II and lipocortin-85) from bovine lung. The procedure yields
milligram quantities of all three lipocortins. Using antisera against lipocortin I and lipocortin
II, purified proteins show no cross contaminations. All forms of lipocortin exhibit equal
potency as in vitro bovine pancreatic phospholipase A 2 inhibitors. Protein kinase C
catalyzes the in, vivo incorporation of about 1.0, 0.7 and 0.4 mole of phosphate per mole of …