An improved coupled test procedure for glutathione peroxidase (EC 1.11. 1.9.) in blood

WA Günzler, H Kremers, L Flohe - 1974 - degruyter.com
WA Günzler, H Kremers, L Flohe
1974degruyter.com
An optimized coupled test system for glutathione peroxidase in red blood cells is presented.
In the improved assay, tert. butyl hydroperoxide was employed as an acceptor substrate at
twenty times Km (app) concentration. By aid of a batching procedure for instant separation of
hemoglobin from glutathione peroxidase, it could be proved that cyanmethemoglobin in the
modified procedure does not affect the spontaneous reaction rate. Further possible
interference with the determination could be excluded. Glutathione peroxidase activities in …
An optimized coupled test system for glutathione peroxidase in red blood cells is presented. In the improved assay, tert. butyl hydroperoxide was employed as an acceptor substrate at twenty times Km (app) concentration. By aid of a batching procedure for instant separation of hemoglobin from glutathione peroxidase, it could be proved that cyanmethemoglobin in the modified procedure does not affect the spontaneous reaction rate. Further possible interference with the determination could be excluded. Glutathione peroxidase activities in red blood cells of normal adults were measured by the improved test method. A glutathione peroxidase activity in human plasma was observed.
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