[HTML][HTML] Glucose and tolbutamide induce apoptosis in pancreatic β-cells: a process dependent on intracellular Ca2+ concentration
IB Efanova, SV Zaitsev, B Zhivotovsky… - Journal of Biological …, 1998 - ASBMB
High concentrations of glucose are considered to be toxic for the pancreatic β-cell. However,
the mechanisms underlying β-cell dysfunction and resulting cell death are not fully
characterized. In the present study we have demonstrated that incubation of pancreatic islets
and β-cells from ob/ob mice and Wistar rats with glucose induced a process of apoptotic β-
cell death, as shown by DNA laddering, TdT-mediated dUTP-biotin nick end-labeling
(TUNEL) technique, and by using DNA-staining dye HOECHST 33342. The obtained results …
the mechanisms underlying β-cell dysfunction and resulting cell death are not fully
characterized. In the present study we have demonstrated that incubation of pancreatic islets
and β-cells from ob/ob mice and Wistar rats with glucose induced a process of apoptotic β-
cell death, as shown by DNA laddering, TdT-mediated dUTP-biotin nick end-labeling
(TUNEL) technique, and by using DNA-staining dye HOECHST 33342. The obtained results …