[HTML][HTML] Monoclonal Antibody 9EG7 Defines a Novel β1 Integrin Epitope Induced by Soluble Ligand and Manganese, but Inhibited by Calcium (∗)
G Bazzoni, DT Shih, CA Buck, ME Hemler - Journal of Biological Chemistry, 1995 - ASBMB
The monoclonal antibody 9EG7 has been previously found to recognize an epitope induced
by manganese on the integrin β 1 chain (Lenter, M., Uhlig, H., Hamann, A., Jeno, P., Imhof,
B., and Vestweber, D.(1993) Proc. Natl. Acad. Sci. USA 90, 9051-9055). Here we show that
treatment of β 1 integrins with manganese or soluble integrin ligands (eg fibronectin and
RGD peptide) induced the 9EG7 epitope. This epitope was also induced upon EGTA
treatment to remove calcium, and the addition of calcium inhibited 9EG7 epitope induction …
by manganese on the integrin β 1 chain (Lenter, M., Uhlig, H., Hamann, A., Jeno, P., Imhof,
B., and Vestweber, D.(1993) Proc. Natl. Acad. Sci. USA 90, 9051-9055). Here we show that
treatment of β 1 integrins with manganese or soluble integrin ligands (eg fibronectin and
RGD peptide) induced the 9EG7 epitope. This epitope was also induced upon EGTA
treatment to remove calcium, and the addition of calcium inhibited 9EG7 epitope induction …