GA binding protein regulates interleukin 7 receptor α-chain gene expression in T cells
HH Xue, J Bollenbacher, V Rovella, R Tripuraneni… - Nature …, 2004 - nature.com
Nature immunology, 2004•nature.com
The interleukin 7 receptor α-chain (IL-7Rα) is essential for T cell development in both
humans and mice and for B cell development in mice. Whereas the transcription factor PU. 1
regulates IL-7Rα expression in mouse pro–B cells via a GGAA motif, we demonstrate here
that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-
7Rα expression in T cells, where PU. 1 is not expressed. Moreover, IL-7Rα expression was
diminished substantially in thymocytes but was normal on B220+ fetal liver cells from mouse …
humans and mice and for B cell development in mice. Whereas the transcription factor PU. 1
regulates IL-7Rα expression in mouse pro–B cells via a GGAA motif, we demonstrate here
that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-
7Rα expression in T cells, where PU. 1 is not expressed. Moreover, IL-7Rα expression was
diminished substantially in thymocytes but was normal on B220+ fetal liver cells from mouse …
Abstract
The interleukin 7 receptor α-chain (IL-7Rα) is essential for T cell development in both humans and mice and for B cell development in mice. Whereas the transcription factor PU.1 regulates IL-7Rα expression in mouse pro–B cells via a GGAA motif, we demonstrate here that GA binding protein (GABP) bound to this site and was essential in the regulation of IL-7Rα expression in T cells, where PU.1 is not expressed. Moreover, IL-7Rα expression was diminished substantially in thymocytes but was normal on B220+ fetal liver cells from mouse embryos with diminished expression of GABPα. Thus, GABP is essential for the regulation of IL-7Rα expression in T cells, and the differential regulation of IL-7Rα in distinct lymphoid lineages is achieved at least in part by differential recruitment of factors to the same GGAA motif.
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