Cdc42 plays a critical role in assembly of sarcomere units in series of cardiac myocytes
T Nagai, M Tanaka-Ishikawa, R Aikawa… - Biochemical and …, 2003 - Elsevier
T Nagai, M Tanaka-Ishikawa, R Aikawa, H Ishihara, W Zhu, Y Yazaki, R Nagai, I Komuro
Biochemical and biophysical research communications, 2003•ElsevierCardiomyocyte hypertrophy is observed in various cardiovascular diseases and causes
heart failure. We here examined the role of small GTP-binding proteins of Rho family in
phenylephrine (PE)-or leukocyte inhibitory factor (LIF)-induced hypertrophic morphogenesis
of cultured neonatal rat cardiomyocytes. Both LIF and PE increased cell size of
cardiomyocytes. LIF induced an increase in the length/width ratio of cardiomyocytes, while
PE did not change the ratio. Adenoviral gene transfer of constitutively active mutants of …
heart failure. We here examined the role of small GTP-binding proteins of Rho family in
phenylephrine (PE)-or leukocyte inhibitory factor (LIF)-induced hypertrophic morphogenesis
of cultured neonatal rat cardiomyocytes. Both LIF and PE increased cell size of
cardiomyocytes. LIF induced an increase in the length/width ratio of cardiomyocytes, while
PE did not change the ratio. Adenoviral gene transfer of constitutively active mutants of …
Cardiomyocyte hypertrophy is observed in various cardiovascular diseases and causes heart failure. We here examined the role of small GTP-binding proteins of Rho family in phenylephrine (PE)-or leukocyte inhibitory factor (LIF)-induced hypertrophic morphogenesis of cultured neonatal rat cardiomyocytes. Both LIF and PE increased cell size of cardiomyocytes. LIF induced an increase in the length/width ratio of cardiomyocytes, while PE did not change the ratio. Adenoviral gene transfer of constitutively active mutants of Cdc42 increased the length/width ratio of cardiomyocytes and dominant negative mutants of Cdc42 conversely inhibited LIF-induced cell-elongation, while mutants of RhoA and Rac1 did not affect the length/width ratio of cardiomyocytes. These results suggest that Cdc42, but not RhoA and Rac1, is involved in LIF-induced sarcomere assembly in series in cardiomyocytes.
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