CHO glycosylation mutants: GPI anchor
Y Maeda, H Ashida, T Kinoshita - Methods in enzymology, 2006 - Elsevier
Glycosylphosphatidylinositol (GPI) is used for anchoring many cell surface proteins to the
plasma membrane. Biosynthesis of GPI anchor, its attachment to proteins, and modification
of GPI‐anchored proteins (GPI‐APs) en route to the plasma membrane are complex
processes (Ferguson, 1999; Kinoshita and Inoue, 2000). GPI‐AP–defective mutant cell lines
derived from CHO and other cells have been very useful in elucidating GPI biosynthetic
pathway and cloning genes involved in these processes. In this chapter, we overview GPI …
plasma membrane. Biosynthesis of GPI anchor, its attachment to proteins, and modification
of GPI‐anchored proteins (GPI‐APs) en route to the plasma membrane are complex
processes (Ferguson, 1999; Kinoshita and Inoue, 2000). GPI‐AP–defective mutant cell lines
derived from CHO and other cells have been very useful in elucidating GPI biosynthetic
pathway and cloning genes involved in these processes. In this chapter, we overview GPI …