[HTML][HTML] Cellular prion protein sensitizes neurons to apoptotic stimuli through Mdm2-regulated and p53-dependent caspase 3-like activation
E Paitel, R Fahraeus, F Checler - Journal of Biological Chemistry, 2003 - ASBMB
We examined the influence of cellular prion protein (PrP c) in the control of cell death in
stably transfected TSM1 cells. PrP c expression enhanced staurosporine-stimulated
neuronal toxicity and DNA fragmentation, caspase 3-like activity and immunoreactivity, and
p53 immunoreactivity and transcriptional activities. Caspase activation was reduced by the
chemical inhibitor of p53, pifithrin-α, as well as by PrP c-or p53-antisense approaches but
remained insensitive to the Fyn kinase inhibitor PP2 (4-amino-5-(4-chloro-phenyl)-7-(t-butyl) …
stably transfected TSM1 cells. PrP c expression enhanced staurosporine-stimulated
neuronal toxicity and DNA fragmentation, caspase 3-like activity and immunoreactivity, and
p53 immunoreactivity and transcriptional activities. Caspase activation was reduced by the
chemical inhibitor of p53, pifithrin-α, as well as by PrP c-or p53-antisense approaches but
remained insensitive to the Fyn kinase inhibitor PP2 (4-amino-5-(4-chloro-phenyl)-7-(t-butyl) …