[HTML][HTML] Biologically Active Recombinant Human Progastrin6–80Contains a Tightly Bound Calcium Ion
GS Baldwin, F Hollande, Z Yang, Y Karelina… - Journal of Biological …, 2001 - ASBMB
Evidence is accumulating that gastrin precursors may act as growth factors for the colonic
mucosa in vivo. The aims of this study were to prepare recombinant human progastrin 6–80
and to investigate its structure and biological activities in vitro. Human progastrin 6–80 was
expressed in Escherichia coli as a glutathione S-transferase fusion protein. After thrombin
cleavage progastrin 6–80 was purified by reverse phase high pressure liquid
chromatography and characterized by radioimmunoassay, amino acid sequencing, and …
mucosa in vivo. The aims of this study were to prepare recombinant human progastrin 6–80
and to investigate its structure and biological activities in vitro. Human progastrin 6–80 was
expressed in Escherichia coli as a glutathione S-transferase fusion protein. After thrombin
cleavage progastrin 6–80 was purified by reverse phase high pressure liquid
chromatography and characterized by radioimmunoassay, amino acid sequencing, and …