Culture of retinal capillary cells using selective growth media

JD Gitlin, PA D'Amore - Microvascular research, 1983 - Elsevier
Capillary fragments are isolated from the microvasculature of the bovine retina using limited
collagenase digestion and sieving. The endothelial cells obtained from these capillary
fragments are cultured in media containing platelet-poor plasma supplemented with retinal
extract, which contains an endothelial cell mitogen. These cells form typical monolayers in
vitro, contain Factor VIII antigen, and can be passaged serially. The pericytes obtained from
these capillary fragments are cultured in media containing calf serum. These cells can also …