During the last few years it has become apparent that ceramide, an intermediary in
sphingolipid metabolism, is an important intracellular mediator of stress responses. This has
led to widespread interest in its function by investigators from diverse fields of study and has
underscored the importance of a rapid and quantitative assay for ceramide
determination.Several methods have been developed for quantifying ceramide including
mass spectrometry and HPLC, both of which require lengthy periods of processing and/or …