[HTML][HTML] In NIH-3T3 fibroblasts, insulin receptor interaction with specific protein kinase C isoforms controls receptor intracellular routing
P Formisano, C Miele, M Caruso, R Auricchio… - Journal of Biological …, 1998 - ASBMB
Insulin increased protein kinase C (PKC) activity by 2-fold in both membrane preparations
and insulin receptor (IR) antibody precipitates from NIH-3T3 cells expressing human IRs
(3T3 hIR). PKC-α,-δ, and-ζ were barely detectable in IR antibody precipitates of unstimulated
cells, while increasing by 7-, 3.5-, and 3-fold, respectively, after insulin addition.
Preexposure of 3T3 hIR cells to staurosporine reduced insulin-induced receptor
coprecipitation with PKC-α,-δ, and-ζ by 3-, 4-, and 10-fold, respectively, accompanied by a …
and insulin receptor (IR) antibody precipitates from NIH-3T3 cells expressing human IRs
(3T3 hIR). PKC-α,-δ, and-ζ were barely detectable in IR antibody precipitates of unstimulated
cells, while increasing by 7-, 3.5-, and 3-fold, respectively, after insulin addition.
Preexposure of 3T3 hIR cells to staurosporine reduced insulin-induced receptor
coprecipitation with PKC-α,-δ, and-ζ by 3-, 4-, and 10-fold, respectively, accompanied by a …