Mouse model for lung tumorigenesis through Cre/lox controlled sporadic activation of the K-Ras oncogene
R Meuwissen, SC Linn, M van der Valk, WJ Mooi… - Oncogene, 2001 - nature.com
R Meuwissen, SC Linn, M van der Valk, WJ Mooi, A Berns
Oncogene, 2001•nature.comThe onset of human lung cancer occurs through sequential mutations in oncogenes and
tumor suppressor genes. Mutations in K-Ras play a prominent role in human non-small cell
lung cancer. We have developed a mouse lung tumor model in which K-Ras can be
sporadically activated through Cre-lox mediated somatic recombination. Adenoviral
mediated delivery of Cre recombinase in lung epithelial cells gave rise to rapid onset of
tumorigenesis, yielding pulmonary adenocarcinomas with 100% incidence after a short …
tumor suppressor genes. Mutations in K-Ras play a prominent role in human non-small cell
lung cancer. We have developed a mouse lung tumor model in which K-Ras can be
sporadically activated through Cre-lox mediated somatic recombination. Adenoviral
mediated delivery of Cre recombinase in lung epithelial cells gave rise to rapid onset of
tumorigenesis, yielding pulmonary adenocarcinomas with 100% incidence after a short …
Abstract
The onset of human lung cancer occurs through sequential mutations in oncogenes and tumor suppressor genes. Mutations in K-Ras play a prominent role in human non-small cell lung cancer. We have developed a mouse lung tumor model in which K-Ras can be sporadically activated through Cre-lox mediated somatic recombination. Adenoviral mediated delivery of Cre recombinase in lung epithelial cells gave rise to rapid onset of tumorigenesis, yielding pulmonary adenocarcinomas with 100% incidence after a short latency. The lung tumor lesions shared many features with human non-small cell lung cancer. Our data show that sporadic expression of the K-Ras oncogene is sufficient to elicit lung tumorigenesis. Therefore this model has many advantages over conventional transgenic models used thus far.
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