A rapid increase in the tyrosine phosphorylation of focal adhesion kinase (FAK) has been extensively documented in cells stimulated by multiple signaling molecules, but very little is known about the regulation of FAK phosphorylation at serine residues. Stimulation of Swiss 3T3 cells with platelet‐derived growth factor (PDGF) promoted a striking increase in the phosphorylation of FAK at Ser‐910, as revealed by site‐specific antibodies that recognized the phosphorylated state of this residue. FAK phosphorylation at Ser‐910 could be distinguished from that at Tyr‐397 in terms of dose‐response relationships and kinetics. Furthermore, the selective phosphoinositide 3‐kinase (PI 3‐kinase) inhibitors wortmannin and LY 294002 abrogated FAK phosphorylation at Tyr‐397 but did not interfere with PDGF‐induced FAK phosphorylation at Ser‐910. Conversely, treatment with U0126, a potent inhibitor of MEK‐mediated ERK activation, prevented FAK phosphorylation at Ser‐910 induced by PDGF but did not interfere with PDGF‐induced FAK phosphorylation at Tyr‐397. These results were extended using growth factors that either stimulate, fibroblast growth factor (FGF), or do not stimulate (insulin) the ERK pathway activation in Swiss 3T3 cells. FGF but not insulin promoted a striking ERK‐dependent phosphorylation of FAK at Ser‐910. Our results indicate that FAK phosphorylation at Tyr‐397 and FAK phosphorylation at Ser‐910 are induced in response to PDGF stimulation through different signaling pathways, namely PI 3‐kinase and ERK, respectively. © 2004 Wiley‐Liss, Inc.