[HTML][HTML] The recruitment of Raf-1 to membranes is mediated by direct interaction with phosphatidic acid and is independent of association with Ras
MA Rizzo, K Shome, SC Watkins, G Romero - Journal of Biological …, 2000 - ASBMB
The serine/threonine kinase Raf-1 is an essential component of the MAPK cascade.
Activation of Raf-1 by extracellular signals is initiated by association with intracellular
membranes. Recruitment of Raf-1 to membranes has been reported to be mediated by direct
association with Ras and by the phospholipase D product phosphatidic acid (PA). Here we
report that insulin stimulation of HIRcB fibroblasts leads to accumulation of Ras, Raf-1,
phosphorylated MEK, phosphorylated MAPK, and PA on endosomal membranes. Mutations …
Activation of Raf-1 by extracellular signals is initiated by association with intracellular
membranes. Recruitment of Raf-1 to membranes has been reported to be mediated by direct
association with Ras and by the phospholipase D product phosphatidic acid (PA). Here we
report that insulin stimulation of HIRcB fibroblasts leads to accumulation of Ras, Raf-1,
phosphorylated MEK, phosphorylated MAPK, and PA on endosomal membranes. Mutations …