Several leukocyte populations have been described within the pregnant mouse uterus, some of which express the integrin beta 7 (ITGB7). Here we demonstrate that the majority of the ITGB7⁺ decidual leukocytes belong to the dendritic cell (DC) lineage. By multiparameter flow cytometric analysis we demonstrated the existence of three distinct DC subsets, characterized by differential expression of ITGA4/ITGB7 (formerly alpha4beta7-integrin) and ITGAE/ITGB7 (formerly alphaEbeta7-integrin). Importantly, the predominant DC subsets reside in distinct microdomains of the Day 9 pregnant mouse uterus. ITGAX⁺ ITGAM^med ITGA4/ITGB7⁺ ITGAE⁻ (formerly CD11c⁺ CD11b^med alpha4beta7⁺ alphaE⁻) cells represent the majority of DCs in the vascular zone (VZ), whereas ITGAX⁺ ITGAM⁻ ITGAE/ITGB7⁺ (formerly CD11c⁺ CD11b⁻ alphaEbeta7⁺) DCs are mainly located in the lower central decidua basalis (cDB) and the underlying myometrium. A population of ITGAX⁺ ITGAM^low DCs lacking ITGB7 are restricted to the cDB. Confocal microscopy studies show direct contact of VZ DCs with uterine natural killer (uNK) cells, suggesting a functional relationship between both cell populations. Collectively, our data identify three phenotypically distinct DC subsets residing in distinct microdomains of the uterus. The differential expression of ITGA4/ITGB7 and ITGAE/ITGB7 suggests distinct functional roles of the different DC subsets during early pregnancy.