[HTML][HTML] A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein: limited co-assembly in vitro …
PM Steinert, YH Chou, V Prahlad, DAD Parry… - Journal of Biological …, 1999 - ASBMB
BHK-21 fibroblasts contain type III vimentin/desmin intermediate filament (IF) proteins that
typically co-isolate and co-cycle in in vitro experiments with certain high molecular weight
proteins. Here, we report purification of one of these and demonstrate that it is in fact the type
VI IF protein nestin. Nestin is expressed in several fibroblastic but not epithelioid cell lines.
We show that nestin forms homodimers and homotetramers but does not form IF by itself in
vitro. In mixtures, nestin preferentially co-assembles with purified vimentin or the type IV IF …
typically co-isolate and co-cycle in in vitro experiments with certain high molecular weight
proteins. Here, we report purification of one of these and demonstrate that it is in fact the type
VI IF protein nestin. Nestin is expressed in several fibroblastic but not epithelioid cell lines.
We show that nestin forms homodimers and homotetramers but does not form IF by itself in
vitro. In mixtures, nestin preferentially co-assembles with purified vimentin or the type IV IF …