Interaction between the keratin cytoskeleton and eEF1Bγ affects protein synthesis in epithelial cells
Nature structural & molecular biology, 2007•nature.com
Abstract Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation
during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles,
keratin intermediate filaments. Disrupting this interaction depresses translation by∼ 20%
and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by
RNA interference–mediated silencing of eEF1Bγ. These findings extend the emerging
relationship between keratin proteins and the translational machinery.
during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles,
keratin intermediate filaments. Disrupting this interaction depresses translation by∼ 20%
and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by
RNA interference–mediated silencing of eEF1Bγ. These findings extend the emerging
relationship between keratin proteins and the translational machinery.
Abstract
Eukaryotic elongation factor-1 (eEF1) is essential for peptide-chain elongation during translation. We report that its γ subunit (eEF1Bγ) specifically binds, and bundles, keratin intermediate filaments. Disrupting this interaction depresses translation by ∼20% and selectively increases 80S ribosomes in epithelial cells, an outcome recapitulated by RNA interference–mediated silencing of eEF1Bγ. These findings extend the emerging relationship between keratin proteins and the translational machinery.
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