[HTML][HTML] The role of N-glycosylation in function and surface trafficking of the human dopamine transporter
LB Li, N Chen, S Ramamoorthy, L Chi, XN Cui… - Journal of Biological …, 2004 - ASBMB
The present study addressed the role of N-linked glycosylation of the human dopamine
transporter (DAT) in its function with the help of mutants, in which canonical N-glycosylation
sites have been removed (N181Q, N181Q, N188Q, and N181Q, N188Q, N205Q), expressed
in human embryonic kidney-293 cells. Removal of canonical sites produced lower molecular
weight species as did enzymatic deglycosylation or blockade of glycosylation, and all three
canonical sites were found to carry sugars. Prevention of N-glycosylation reduced both …
transporter (DAT) in its function with the help of mutants, in which canonical N-glycosylation
sites have been removed (N181Q, N181Q, N188Q, and N181Q, N188Q, N205Q), expressed
in human embryonic kidney-293 cells. Removal of canonical sites produced lower molecular
weight species as did enzymatic deglycosylation or blockade of glycosylation, and all three
canonical sites were found to carry sugars. Prevention of N-glycosylation reduced both …