Expression of adhesion molecules in cultured human pulmonary microvascular endothelial cells
J Shen, RG Ham, S Karmiol - Microvascular research, 1995 - Elsevier
HPMECs were successfully isolated by differential trypsinization from peripheral lung lobes.
The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more
than 20 passages (1: 4 split ratio) in vitro. Cells were characterized as endothelial based
upon their cobblestone morphology, the presence of factor VIII-related antigen, incorporation
of Dil-Ac-LDL, tubule-like structure formation in Matrigel, and positive staining for ACE.
Adhesion molecules were tested at passage 3 and passage 12. Cells demonstrated intense …
The cells proliferated rapidly in EGM-MV with 10% FBS and were serially cultivated for more
than 20 passages (1: 4 split ratio) in vitro. Cells were characterized as endothelial based
upon their cobblestone morphology, the presence of factor VIII-related antigen, incorporation
of Dil-Ac-LDL, tubule-like structure formation in Matrigel, and positive staining for ACE.
Adhesion molecules were tested at passage 3 and passage 12. Cells demonstrated intense …