The use of recombinant T cell receptors (TCRs) to target therapeutic interventions has been
hindered by the naturally low affinity of TCR interactions with peptide major
histocompatibility complex ligands. Here, we use multimeric forms of soluble heterodimeric
αβ TCRs for specific detection of target cells pulsed with cognate peptide, discrimination of
quantitative changes in antigen display at the cell surface, identification of virus-infected
cells, inhibition of antigen-specific cytotoxic T lymphocyte activation, and identification of …