A new method for detecting TNF-α-secreting cells using direct-immunofluorescence surface membrane stainings

C Bueno, A Rodriguez-Caballero… - Journal of …, 2002 - Elsevier
In the present study, a new flow cytometric method for the identification of TNF-α-secreting
cells based on the use of a TNF-α converting enzyme (TACE) inhibitor compound (BB3103)
is described. TNF-α secreting cells were measured in parallel in stimulated peripheral blood
samples (n= 4), using the BB3103 TACE inhibitor or brefeldin A as secretion blocking
agents. To induce TNF-α production by PB T-cells and monocytes, whole blood samples
were stimulated either for 4 h with PMA plus ionomycin or for 6 h with LPS plus IFNγ …