[HTML][HTML] Phosphorylation Modulates the Function of the Calcium Release Channel of Sarcoplasmic Reticulum from Cardiac Muscle (∗)
J Hain, H Onoue, M Mayrleitner, S Fleischer… - Journal of Biological …, 1995 - ASBMB
The cardiac calcium release channel (CRC) of sarcoplasmic reticulum vesicles was
incorporated into planar lipid membranes to evaluate modulation of channel activity by
phosphorylation and dephosphorylation. For this purpose a microsyringe application directly
to the membrane was used to achieve sequential and multiple treatments of channels with
highly purified kinases and phosphatases. Cyclic application of protein kinase A (PKA) or
Ca/calmodulin-dependent protein kinase II (CalPK) and potato acid phosphatase or protein …
incorporated into planar lipid membranes to evaluate modulation of channel activity by
phosphorylation and dephosphorylation. For this purpose a microsyringe application directly
to the membrane was used to achieve sequential and multiple treatments of channels with
highly purified kinases and phosphatases. Cyclic application of protein kinase A (PKA) or
Ca/calmodulin-dependent protein kinase II (CalPK) and potato acid phosphatase or protein …