[HTML][HTML] Insig required for sterol-mediated inhibition of Scap/SREBP binding to COPII proteins in vitro*♦
LP Sun, L Li, JL Goldstein, MS Brown - Journal of Biological Chemistry, 2005 - ASBMB
When added to living cells, sterols such as cholesterol and 25-hydroxycholesterol block the
lateral movement of sterol regulatory element-binding proteins (SREBPs) into COPII-coated
vesicles on endoplasmic reticulum (ER) membranes and thereby prevent the SREBPs from
reaching the Golgi complex for processing to the mature forms that activate cholesterol
synthesis. Sorting of SREBPs into COPII vesicles is mediated by Sar1 and the coat proteins
Sec23 and Sec24. Here, we explore the mechanism of sterol inhibition in vitro through use …
lateral movement of sterol regulatory element-binding proteins (SREBPs) into COPII-coated
vesicles on endoplasmic reticulum (ER) membranes and thereby prevent the SREBPs from
reaching the Golgi complex for processing to the mature forms that activate cholesterol
synthesis. Sorting of SREBPs into COPII vesicles is mediated by Sar1 and the coat proteins
Sec23 and Sec24. Here, we explore the mechanism of sterol inhibition in vitro through use …