[HTML][HTML] β2-adrenergic receptor signaling and desensitization elucidated by quantitative modeling of real time cAMP dynamics
JD Violin, LM DiPilato, N Yildirim, TC Elston… - Journal of biological …, 2008 - ASBMB
G protein-coupled receptor signaling is dynamically regulated by multiple feedback
mechanisms, which rapidly attenuate signals elicited by ligand stimulation, causing
desensitization. The individual contributions of these mechanisms, however, are poorly
understood. Here, we use an improved fluorescent biosensor for cAMP to measure second
messenger dynamics stimulated by endogenous β 2-adrenergic receptor (β 2 AR) in living
cells. β 2 AR stimulation with isoproterenol results in a transient pulse of cAMP, reaching a …
mechanisms, which rapidly attenuate signals elicited by ligand stimulation, causing
desensitization. The individual contributions of these mechanisms, however, are poorly
understood. Here, we use an improved fluorescent biosensor for cAMP to measure second
messenger dynamics stimulated by endogenous β 2-adrenergic receptor (β 2 AR) in living
cells. β 2 AR stimulation with isoproterenol results in a transient pulse of cAMP, reaching a …