Rapid fluorometric assay of LDL receptor activity by DiI-labeled LDL.
ZF Stephan, EC Yurachek - Journal of lipid research, 1993 - ASBMB
DiI-LDL (3, 3'-dioctadecylindocarbocyanine-low density lipoprotein) has been extensively
used in morphological and microscopic studies of receptor-mediated metabolism of LDL in
many cell lines. To date the use of this fluorescent probe in a quantitative assay of LDL
receptor activity has not been widely used in studies with multiple samples due to the lack of
a practical method for quantitatively recovering cell-associated DiI. Therefore, detection by
125I-labeled LDL has remained the method of choice for assaying LDL receptor activity …
used in morphological and microscopic studies of receptor-mediated metabolism of LDL in
many cell lines. To date the use of this fluorescent probe in a quantitative assay of LDL
receptor activity has not been widely used in studies with multiple samples due to the lack of
a practical method for quantitatively recovering cell-associated DiI. Therefore, detection by
125I-labeled LDL has remained the method of choice for assaying LDL receptor activity …