[HTML][HTML] Identification of a PKB/Akt Hydrophobic Motif Ser-473 Kinase as DNA-dependent Protein Kinase*♦
Full activation of protein kinase B (PKB)/Akt requires phosphorylation on Thr-308 and Ser-
473 by 3-phosphoinositide-dependent kinase-1 (PDK1) and Ser-473 kinase (S473K),
respectively. Although PDK1 has been well characterized, the identification of the S473K
remains controversial. A major PKB Ser-473 kinase activity was purified from the membrane
fraction of HEK293 cells and found to be DNA-dependent protein kinase (DNA-PK). DNA-PK
co-localized and associated with PKB at the plasma membrane. In vitro, DNA-PK …
473 by 3-phosphoinositide-dependent kinase-1 (PDK1) and Ser-473 kinase (S473K),
respectively. Although PDK1 has been well characterized, the identification of the S473K
remains controversial. A major PKB Ser-473 kinase activity was purified from the membrane
fraction of HEK293 cells and found to be DNA-dependent protein kinase (DNA-PK). DNA-PK
co-localized and associated with PKB at the plasma membrane. In vitro, DNA-PK …