[20] Role of 1α, 25-dihydroxyvitamin D3 in osteoclast differentiation and function
T Suda, E Jimi, I Nakamura, N Takahashi - Methods in enzymology, 1997 - Elsevier
Publisher Summary This chapter describes the methods for examining osteoclast
development and function. Cytochemical staining for tartrate-resistant acid phosphatase
(TRAP) is widely used for identifying osteoclasts in vivo and in vitro. Osteoclasts are shown
to possess abundant calcitonin receptors. Expression of calcitonin receptors is one of the
most reliable markers for identifying osteoclasts. For autoradiography of 125 I-labeled
calcitonin, cultures are performed on plastic coverslips placed in 24-well culture plates …
development and function. Cytochemical staining for tartrate-resistant acid phosphatase
(TRAP) is widely used for identifying osteoclasts in vivo and in vitro. Osteoclasts are shown
to possess abundant calcitonin receptors. Expression of calcitonin receptors is one of the
most reliable markers for identifying osteoclasts. For autoradiography of 125 I-labeled
calcitonin, cultures are performed on plastic coverslips placed in 24-well culture plates …