[HTML][HTML] Mutants in the Putative Nucleotide-binding Region of the Plasma Membrane Ca2+-Pump: A REDUCTION IN ACTIVITY DUE TO SLOW …
Mutants of individual residues of the plasma membrane Ca 2+-pump were made in the
highly conserved region that (in related P-type ATPases) has been associated with
nucleotide binding. Alteration of the strictly conserved Asp 672 to Glu nearly eliminated the
ability of the pump to transport Ca 2+, while alteration at Val 674, Arg 675, and Lys 686
reduced the activity. High levels of ATP (25 mM) did not overcome the reduced activity,
indicating that it could not be due to a reduction in the affinity for ATP. Effects not directly …
highly conserved region that (in related P-type ATPases) has been associated with
nucleotide binding. Alteration of the strictly conserved Asp 672 to Glu nearly eliminated the
ability of the pump to transport Ca 2+, while alteration at Val 674, Arg 675, and Lys 686
reduced the activity. High levels of ATP (25 mM) did not overcome the reduced activity,
indicating that it could not be due to a reduction in the affinity for ATP. Effects not directly …