[HTML][HTML] Diverse effects of pathogenic mutations of Parkin that catalyze multiple monoubiquitylation in vitro
N Matsuda, T Kitami, T Suzuki, Y Mizuno… - Journal of Biological …, 2006 - ASBMB
Mutational dysfunction of PARKIN gene, which encodes a double RING finger protein and
has ubiquitin ligase E3 activity, is the major cause of autosomal recessive juvenile
Parkinsonism. Although many studies explored the functions of Parkin, its biochemical
character is poorly understood. To address this issue, we established an E3 assay system
using maltose-binding protein-fused Parkin purified from Escherichia coli. Using this
recombinant Parkin, we found that not the front but the rear RING finger motif is responsible …
has ubiquitin ligase E3 activity, is the major cause of autosomal recessive juvenile
Parkinsonism. Although many studies explored the functions of Parkin, its biochemical
character is poorly understood. To address this issue, we established an E3 assay system
using maltose-binding protein-fused Parkin purified from Escherichia coli. Using this
recombinant Parkin, we found that not the front but the rear RING finger motif is responsible …