[HTML][HTML] Separation of “glycosphingolipid signaling domain” from caveolin-containing membrane fraction in mouse melanoma B16 cells and its role in cell adhesion …
K Iwabuchi, K Handa, S Hakomori - Journal of Biological Chemistry, 1998 - ASBMB
Two membrane subfractions, one enriched in GM3 ganglioside and the other containing
caveolin, were separated from low density detergent-insoluble membrane fraction prepared
by sucrose density gradient centrifugation of postnuclear fraction of mouse melanoma B16
cells. The GM3-enriched subfraction, separated by anti-GM3 monoclonal antibody DH2,
contained sphingomyelin, cholesterol, c-Src, and Rho A but not caveolin. In contrast, the
caveolin-containing subfraction, separated by anti-caveolin antibody, contained neither …
caveolin, were separated from low density detergent-insoluble membrane fraction prepared
by sucrose density gradient centrifugation of postnuclear fraction of mouse melanoma B16
cells. The GM3-enriched subfraction, separated by anti-GM3 monoclonal antibody DH2,
contained sphingomyelin, cholesterol, c-Src, and Rho A but not caveolin. In contrast, the
caveolin-containing subfraction, separated by anti-caveolin antibody, contained neither …