Mutations to Ku reveal differences in human somatic cell lines
KR Fattah, BL Ruis, EA Hendrickson - DNA repair, 2008 - Elsevier
NHEJ (non-homologous end joining) is the predominant mechanism for repairing DNA
double-stranded breaks in human cells. One essential NHEJ factor is the Ku heterodimer,
which is composed of Ku70 and Ku86. Here we have generated heterozygous loss-of-
function mutations for each of these genes in two different human somatic cell lines, HCT116
and NALM-6, using gene targeting. Previous work had suggested that phenotypic
differences might exist between the genes and/or between the cell lines. By providing a side …
double-stranded breaks in human cells. One essential NHEJ factor is the Ku heterodimer,
which is composed of Ku70 and Ku86. Here we have generated heterozygous loss-of-
function mutations for each of these genes in two different human somatic cell lines, HCT116
and NALM-6, using gene targeting. Previous work had suggested that phenotypic
differences might exist between the genes and/or between the cell lines. By providing a side …