A stable, radioactive substrate emulsion for assay of lipoprotein lipase

P Nilsson-Ehle, MC Schotz - Journal of lipid research, 1976 - Elsevier
P Nilsson-Ehle, MC Schotz
Journal of lipid research, 1976Elsevier
A method is described for the assay of lipoprotein lipase, using a stable, radioactive
substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in
glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six
weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with
buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in
a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from …
A method is described for the assay of lipoprotein lipase, using a stable, radioactive substrate emulsion. Fatty acid-labeled trioleoylglycerol was emulsified by homogenization in glycerol with lecithin as detergent. This anhydrous emulsion was stable for at least six weeks. Substrate solutions for enzyme assay were prepared by diluting the emulsion with buffer containing serum and albumin. The fatty acid produced on hydrolysis was isolated in a one-step liquid-liquid partition system. Incubations with extracts of acetone powders from adipose tissue displayed characteristics of lipoprotein lipase activity, i.e., serum dependence and inhibition by NaCl and protamine. The method is rapid (<1 hour), sensitive and reproducible, and suitable for routine use.
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