Separation of near full-length hepatitis C virus quasispecies variants from a complex population
D Zhou, X Fan, D Tan, Y Xu, JE Tavis… - Journal of virological …, 2007 - Elsevier
A long RT-PCR (LRP) protocol was developed recently for robust amplification of a near full-
length HCV genomic sequence from clinical samples, followed by efficient cloning [Fan, X.,
Xu, Y., Di Biceglie, AM, 2006. Efficient amplification and cloning of near full-length hepatitis
C virus genome from clinical samples. Biochem. Biophys. Res. Commun. 346, 1163–1172].
In the present study, the LRP protocol has been estimated for its error rate and the validation
by sequencing fully the near full-length HCV inserts from six recombinant clones derived …
length HCV genomic sequence from clinical samples, followed by efficient cloning [Fan, X.,
Xu, Y., Di Biceglie, AM, 2006. Efficient amplification and cloning of near full-length hepatitis
C virus genome from clinical samples. Biochem. Biophys. Res. Commun. 346, 1163–1172].
In the present study, the LRP protocol has been estimated for its error rate and the validation
by sequencing fully the near full-length HCV inserts from six recombinant clones derived …