[HTML][HTML] Mouse down-regulated in adenoma (DRA) is an intestinal Cl−/HCO3− exchanger and is up-regulated in colon of mice lacking the NHE3 Na+/H+ exchanger
JE Melvin, K Park, L Richardson, PJ Schultheis… - Journal of Biological …, 1999 - ASBMB
Mutations in human DRA cause congenital chloride diarrhea, thereby raising the possibility
that it functions as a Cl−/HCO 3− exchanger. To test this hypothesis we cloned a cDNA
encoding mouse DRA (mDRA) and analyzed its activity in cultured mammalian cells. When
expressed in HEK 293 cells, mDRA conferred Na+-independent, electroneutral Cl−/CHO 3−
exchange activity. Removal of extracellular Cl− from medium containing HCO 3− caused a
rapid intracellular alkalinization, whereas the intracellular pH increase following Cl− removal …
that it functions as a Cl−/HCO 3− exchanger. To test this hypothesis we cloned a cDNA
encoding mouse DRA (mDRA) and analyzed its activity in cultured mammalian cells. When
expressed in HEK 293 cells, mDRA conferred Na+-independent, electroneutral Cl−/CHO 3−
exchange activity. Removal of extracellular Cl− from medium containing HCO 3− caused a
rapid intracellular alkalinization, whereas the intracellular pH increase following Cl− removal …