Evaluation of the sensitive step of inhibition of chondrogenesis by retinoids in limb mesenchymal cells in vitro
B Zimmerman, D Tsambaos - Cell differentiation, 1985 - Elsevier
The sensitive step of inhibition of chondrogenesis in vitro by retinoids was investigated in
modified micromass cultures of limb bud mesenchymal cells from mouse embryos of day 11
and 12. Evaluation of chondrogenesis was performed after alcian blue staining, using a
simple random hit counting of cartilage nodules. All-trans-retinoic acid, 13-cis-retinoic acid,
and a newly developed arotinoid, RO 13-6298, were tested for their ability to inhibit
chondrogenesis. We found that inhibition of chondrogenesis depended on the dosage and …
modified micromass cultures of limb bud mesenchymal cells from mouse embryos of day 11
and 12. Evaluation of chondrogenesis was performed after alcian blue staining, using a
simple random hit counting of cartilage nodules. All-trans-retinoic acid, 13-cis-retinoic acid,
and a newly developed arotinoid, RO 13-6298, were tested for their ability to inhibit
chondrogenesis. We found that inhibition of chondrogenesis depended on the dosage and …