Comparison of estrogen receptors in human premenopausal and postmenopausal uteri: indication of biologically inactive receptor in postmenopausal uteri
JH Strathy, CB Coulam, TC Spelsberg - American journal of obstetrics and …, 1982 - Elsevier
Analysis of the estrogen receptor (ER) from uteri of premenopausal and postmenopausal
patients has largely been limited to quantitation. The present study examined the biologic
activity of the receptors with regard to their nuclear binding capacities. A Scatchard analysis
of myometrial cytosol indicated high-affinity and low-capacity estradiol binding (K d= 10− 10
M). A 35% ammonium sulfate fraction of cytosol was characterized for steroid speciflcity,
receptor concentration, isoelectric focusing patterns, and capacity of the ER to bind to …
patients has largely been limited to quantitation. The present study examined the biologic
activity of the receptors with regard to their nuclear binding capacities. A Scatchard analysis
of myometrial cytosol indicated high-affinity and low-capacity estradiol binding (K d= 10− 10
M). A 35% ammonium sulfate fraction of cytosol was characterized for steroid speciflcity,
receptor concentration, isoelectric focusing patterns, and capacity of the ER to bind to …