Age-dependent, ovary-independent decrease in the nuclear binding kinetics of estrogen receptors in the brain of the C57BL6J mouse
S Belisle, D Bellabarba, JG Lehoux - American journal of obstetrics and …, 1985 - Elsevier
To further define the role of aging of the brain in the induction of reproductive acyclicity, we
put to death intact as well as castrated female C57BL 6J mice of various ages before and
from 0.5 to 24 hours after subcutaneous injection of 0.2 μg of 17β-estradiol. Pooled
hypothalamic and pituitary tissues were dissected and cytosolic/nuclear estrogen receptors
were assayed in buffer that consisted of 10 mmol/L Tris (hydroxymethylaminomethane)
hydrochloride, 1.5 mmol/L ethylenediaminetetraacetic acid, and 0.5 mmol/L dithiothreitol …
put to death intact as well as castrated female C57BL 6J mice of various ages before and
from 0.5 to 24 hours after subcutaneous injection of 0.2 μg of 17β-estradiol. Pooled
hypothalamic and pituitary tissues were dissected and cytosolic/nuclear estrogen receptors
were assayed in buffer that consisted of 10 mmol/L Tris (hydroxymethylaminomethane)
hydrochloride, 1.5 mmol/L ethylenediaminetetraacetic acid, and 0.5 mmol/L dithiothreitol …