Analysis of heat-shock related gene expression in head-and-neck cancer using cDNA arrays
N Narita, I Noda, T Ohtsubo, S Fujieda… - International Journal of …, 2002 - Elsevier
Purpose: The aim of the present study was to identify genes regulated in the early response
to heat-shock in human head-and-neck cancer cells using a cDNA array. Methods and
Materials: IMC-3 cells were heat-shocked at 44° C for 30 min, then incubated for 6 h. After 6
h incubation, mRNAs were extracted. Early gene expressions in IMC-3 cells were analyzed
using a cDNA array after heat-shock. In addition, reverse transcription-polymerase chain
reaction (RT-PCR) was performed to identify the expressions of mRNA for confirming …
to heat-shock in human head-and-neck cancer cells using a cDNA array. Methods and
Materials: IMC-3 cells were heat-shocked at 44° C for 30 min, then incubated for 6 h. After 6
h incubation, mRNAs were extracted. Early gene expressions in IMC-3 cells were analyzed
using a cDNA array after heat-shock. In addition, reverse transcription-polymerase chain
reaction (RT-PCR) was performed to identify the expressions of mRNA for confirming …