Injection of a porcine cytosolic sperm factor (SF) or of a porcine testicular extract into mammalian eggs triggers oscillations of intracellular free calcium ([Ca²⁺]_i) similar to those initiated by fertilization. To elucidate whether SF activates the phosphoinositide (PI) pathway, mouse eggs or SF were incubated with U73122, an inhibitor of events leading to phospholipase C (PLC) activation and/or of PLC itself. In both cases, U73122 blocked the ability of SF to induce [Ca²⁺]_i oscillations, although it did not inhibit Ca²⁺ release caused by injection of inositol 1,4,5-triphosphate (IP₃). The inactive analogue, U73343, had no effect on SF-induced Ca²⁺ responses. To determine at the single cell level whether SF triggers IP₃ production concomitantly with a [Ca²⁺]_i rise, SF was injected into Xenopus oocytes and IP₃ concentration was determined using a biological detector cell combined with capillary electrophoresis. Injection of SF induced a significant increase in [Ca²⁺]_i and IP₃ production in these oocytes. Using ammonium sulfate precipitation, chromatographic fractionation, and Western blotting, we determined whether PLCγ1, PLCγ2, or PLCδ4 and/or its splice variants, which are present in sperm and testis, are responsible for the Ca²⁺ activity in the extracts. Our results revealed that active fractions do not contain PLCγ1, PLCγ2, or PLCδ4 and/or its splice variants, which were present in inactive fractions. We also tested whether IP₃ could be the sensitizing stimulus of the Ca²⁺-induced Ca²⁺ release mechanism, which is an important feature of fertilized and SF-injected eggs. Eggs injected with adenophostin A, an IP₃ receptor agonist, showed enhanced Ca²⁺ responses to CaCl₂ injections. Thus, SF, and probably sperm, induces [Ca²⁺]_i rises by persistently stimulating IP₃ production, which in turn results in long-lasting sensitization of Ca²⁺-induced Ca²⁺ release. Whether SF is itself a PLC or whether it acts upstream of the egg’s PLCs remains to be elucidated.