Inflammatory conditions induce gap junctional communication between rat Kupffer cells both in vivo and in vitro
EA Eugenín, HE González, HA Sánchez, MC Brañes… - Cellular …, 2007 - Elsevier
Connexin43 (Cx43), a gap junction protein subunit, has been previously detected in Kupffer
cells (KCs) during liver inflammation, however, KCs phagocytose cell debris that may
include Cx43 protein, which could explain the detection of Cx43 in KCs. We determined that
KCs express Cx43 and form gap junctions (GJs) both in vivo and in vitro. In liver sections of
animals treated with LPS, Cx43 was detected at ED2+ cells interfaces, indicating formation
of GJs between KCs in vivo. In vitro, unstimulated KCs cultures did not form functional GJs …
cells (KCs) during liver inflammation, however, KCs phagocytose cell debris that may
include Cx43 protein, which could explain the detection of Cx43 in KCs. We determined that
KCs express Cx43 and form gap junctions (GJs) both in vivo and in vitro. In liver sections of
animals treated with LPS, Cx43 was detected at ED2+ cells interfaces, indicating formation
of GJs between KCs in vivo. In vitro, unstimulated KCs cultures did not form functional GJs …