[HTML][HTML] Rotenone-like action of the branched-chain phytanic acid induces oxidative stress in mitochondria
P Schönfeld, G Reiser - Journal of biological chemistry, 2006 - ASBMB
Phytanic acid (Phyt) increase is associated with the hereditary neurodegenerative Refsum
disease. To elucidate the still unclear toxicity of Phyt, mitochondria from brain and heart of
adult rats were exposed to free Phyt. Phyt at low micromolar concentrations (maximally: 100
nmol/mg of protein) enhances superoxide (O 2·¯) 2 2The abbreviations used are: O 2·¯,
superoxide; DCF, dichlorofluorescein; DCFH, dichlorofluorescin; MTT, 3-(4, 5-
dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; FCCP, p …
disease. To elucidate the still unclear toxicity of Phyt, mitochondria from brain and heart of
adult rats were exposed to free Phyt. Phyt at low micromolar concentrations (maximally: 100
nmol/mg of protein) enhances superoxide (O 2·¯) 2 2The abbreviations used are: O 2·¯,
superoxide; DCF, dichlorofluorescein; DCFH, dichlorofluorescin; MTT, 3-(4, 5-
dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; FCCP, p …