Cholesterol absorption from the intestine is a major determinant of reverse cholesterol transport from peripheral tissue macrophages
E Sehayek, SL Hazen - Arteriosclerosis, thrombosis, and vascular …, 2008 - Am Heart Assoc
E Sehayek, SL Hazen
Arteriosclerosis, thrombosis, and vascular biology, 2008•Am Heart AssocObjective—We examined the effect of ezetimibe, a cholesterol absorption (CA) inhibitor, and
genetic determinants of CA on reverse cholesterol transport (RCT) from subcutaneously
injected macrophages using a new dual isotope label technique. Methods and Results—
Treatment of C57BL/6J mice with ezetimibe decreased dietary CA by 86% and increased
RCT from peripheral tissue macrophages (PTM) by 6-fold (P< 0.0001). Moreover, congenic
14DKK mice with a modest 41% decrease in dietary CA displayed a 67% increase in RCT …
genetic determinants of CA on reverse cholesterol transport (RCT) from subcutaneously
injected macrophages using a new dual isotope label technique. Methods and Results—
Treatment of C57BL/6J mice with ezetimibe decreased dietary CA by 86% and increased
RCT from peripheral tissue macrophages (PTM) by 6-fold (P< 0.0001). Moreover, congenic
14DKK mice with a modest 41% decrease in dietary CA displayed a 67% increase in RCT …
Objective— We examined the effect of ezetimibe, a cholesterol absorption (CA) inhibitor, and genetic determinants of CA on reverse cholesterol transport (RCT) from subcutaneously injected macrophages using a new dual isotope label technique.
Methods and Results— Treatment of C57BL/6J mice with ezetimibe decreased dietary CA by 86% and increased RCT from peripheral tissue macrophages (PTM) by 6-fold (P<0.0001). Moreover, congenic 14DKK mice with a modest 41% decrease in dietary CA displayed a 67% increase in RCT from PTM (P<0.007).
Conclusions— These findings indicate that pharmacological and genetic modifiers of cholesterol absorption are major determinants of reverse cholesterol transport from peripheral tissue macrophages.
Am Heart Assoc