Functional analysis of highly defined, FACS-isolated populations of human regulatory CD4+ CD25+ T cells
C Baecher-Allan, E Wolf, DA Hafler - Clinical immunology, 2005 - Elsevier
The importance of CD4+ CD25+ regulatory T cells (Treg) in maintaining immune
homeostasis has been directly demonstrated in vivo by their manipulation in a number of
autoimmune disease models in the mouse. In the study of human regulatory cells, we have
found that the cells that consistently demonstrate the in vitro regulatory activity most similar
to that described for murine cells in vitro are best identified by restricting the isolation of
CD25+ CD4 T cells to those cells expressing only the highest levels of CD25, representing …
homeostasis has been directly demonstrated in vivo by their manipulation in a number of
autoimmune disease models in the mouse. In the study of human regulatory cells, we have
found that the cells that consistently demonstrate the in vitro regulatory activity most similar
to that described for murine cells in vitro are best identified by restricting the isolation of
CD25+ CD4 T cells to those cells expressing only the highest levels of CD25, representing …