We aimed to explore the change in level of apelin and its receptor APJ during myocardial
injury and the therapeutic effects of apelin in myocardial injury. Rat myocardial injury was
induced by subcutaneous injection of a high dose of isoproterenol (ISO); apelin and APJ
mRNA levels were determined by RT-PCR; APJ protein was determined by Western blot;
EIA and RIA were used to measure the apelin content and receptor binding, respectively.
Plasma lactate dehydrogenase (LDH) activity and myocardial and plasma malondialdehyde …