Analysis of the immune response against tetanus toxoid: enumeration of specific T helper cells by the Elispot assay
S Mayer, M Laumer, A Mackensen, R Andreesen… - Immunobiology, 2002 - Elsevier
Tetanus toxoid (TT) is an antigen known to induce strong T cell specific immune responses
in humans after vaccination. Here we have used the Elispot assay to assess the number of
TT-specific Interferon-g (IFN-g) secreting T cells present in individuals and monitored the
number of TT specific T cells present in the donors for more than two years. In each of the 22
healthy volunteers tested, TT-specific T cells could be detected. Six out of 7 repetitively
tested donors showed a remarkably constant number of TT-specific IFN-g secreting T cells …
in humans after vaccination. Here we have used the Elispot assay to assess the number of
TT-specific Interferon-g (IFN-g) secreting T cells present in individuals and monitored the
number of TT specific T cells present in the donors for more than two years. In each of the 22
healthy volunteers tested, TT-specific T cells could be detected. Six out of 7 repetitively
tested donors showed a remarkably constant number of TT-specific IFN-g secreting T cells …