Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003

T Horimoto, N Fukuda, K Iwatsuki-Horimoto… - Journal of Veterinary …, 2004 - jstage.jst.go.jp
T Horimoto, N Fukuda, K Iwatsuki-Horimoto, Y Guan, W Lim, M Peiris, S Sugii, T Odagiri…
Journal of Veterinary Medical Science, 2004jstage.jst.go.jp
To assess whether the antigenic properties of H5 hemagglutinin (HA) change over time due
to antigenic drift, we produced a panel of monoclonal antibodies (mAbs) against the HA of
the index H5N1 human influenza A virus, A/Hong Kong/156/97. By immunizing mice with a
plasmid expressing this HA and boosting the initial immunization with cell lysates
transfected with the plasmid, a total of six hybridomas producing HA-specific mAbs were
established: four to the HA1 subunit with hemadsorption-inhibiting activity and two to the …
Abstract
To assess whether the antigenic properties of H5 hemagglutinin (HA) change over time due to antigenic drift, we produced a panel of monoclonal antibodies (mAbs) against the HA of the index H5N1 human influenza A virus, A/Hong Kong/156/97. By immunizing mice with a plasmid expressing this HA and boosting the initial immunization with cell lysates transfected with the plasmid, a total of six hybridomas producing HA-specific mAbs were established: four to the HA1 subunit with hemadsorption-inhibiting activity and two to the HA2 subunit. None of the mAbs to HA1 could bind to the HA of a recent human isolate, A/Hong Kong/213/2003, indicating that there are substantial antigenic differences between the H5N1 human influenza virus isolated in 1997 and that isolate d in 2003.
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