[HTML][HTML] Localization of von Willebrand factor-binding sites for platelet glycoprotein Ib and botrocetin by charged-to-alanine scanning mutagenesis

T Matsushita, D Meyer, JE Sadler - Journal of Biological Chemistry, 2000 - ASBMB
At sites of vascular injury, von Willebrand factor (VWF) mediates platelet adhesion through
binding to platelet glycoprotein Ib (GPIb). Previous studies identified clusters of charged
residues within VWF domain A1 that were involved in binding GPIb or botrocetin. The
contribution of 28 specific residues within these clusters was analyzed by mutating single
amino acids to alanine. Binding to a panel of six conformation-dependent monoclonal
antibodies was decreased by mutations at Asp 514, Asp 520, Arg 552, and Arg 611 …