[HTML][HTML] The repressed nuclear receptor CAR responds to phenobarbital in activating the human CYP2B6 gene

T Sueyoshi, T Kawamoto, I Zelko, P Honkakoski… - Journal of Biological …, 1999 - ASBMB
The endogenous CYP2B6 gene becomes phenobarbital (PB) inducible in androstenol-
treated HepG2 cells either transiently or stably transfected with a nuclear receptor CAR
expression vector. The PB induction mediated by CAR is regulated by a conserved 51-base
pair element called PB-responsive enhancer module (PBREM) that has now been located
between− 1733 and− 1683 bp in the gene's 5′-flanking region. An in vitro translated CAR
acting as a retinoid X receptor α heterodimer binds directly to the two nuclear receptor sites …