[HTML][HTML] Cytoplasmic localization of pregnane X receptor and ligand-dependent nuclear translocation in mouse liver
EJ Squires, T Sueyoshi, M Negishi - Journal of Biological Chemistry, 2004 - ASBMB
The pregnane X receptor (PXR) plays an important role in the response to xenobiotics and
endogenous toxins. We have used a specific anti-PXR antibody in the Western blotting of
mouse liver nuclear extracts to show that PXR is accumulated in the nucleus after treatment
with 5-pregnen-3β-ol-20-one-16α-carbonitrile (PCN), followed by an increase in Cyp3a11
mRNA. Expression of wild type PXR and various mutants as green fluorescent fusion
proteins in mouse livers showed that PXR was retained in the cytoplasm from where PCN …
endogenous toxins. We have used a specific anti-PXR antibody in the Western blotting of
mouse liver nuclear extracts to show that PXR is accumulated in the nucleus after treatment
with 5-pregnen-3β-ol-20-one-16α-carbonitrile (PCN), followed by an increase in Cyp3a11
mRNA. Expression of wild type PXR and various mutants as green fluorescent fusion
proteins in mouse livers showed that PXR was retained in the cytoplasm from where PCN …